Activity T2.

Development of superparamagnetic MRI contrast agents using a core/shell design, with enhanced biocompatibility and low toxicity.

Sub-activity T2.1. Synthesis of nanoparticles (NPs) with iron oxide core and gold shell, capped with polyethylene glycol (PEG), human albumin or glucose.

Sub-activity T2.1.1 Synthesis of core/shell iron oxide/gold NPs capped with PEG, human albumin or glucose.

Sub-activity T2.1.2 Magnetic and physico-chemical characterization of core/shell iron oxide/gold NPs.

Sub-activity T2.1.3 Assessment of NPs interaction with proteins from blood plasma.

Sub-activity T2.2. In vitro studies.

Sub-activity T2.2.1 Viability and cytotoxicity studies.

Sub-activity T2.2.2 NPs intracellular localization.

Sub-activity T2.2.3 In vitro contrast-to-noise ratio evaluation.

Sub-activity T2.3. In vivo studies.

Sub-activity T2.3.1 In vivo contrast-to-noise ratio evaluation.

Sub-activity T2.3.2 In vivo single and repeated dose toxicity study in rat.

Sub-activity T2.3.3 Inflammatory response to core/shell iron oxide/gold NPs.

Results

  • Protocol for synthesis and physico-chemical characterization of nanoparticles
  • Protocol for gold reduction and for production of stable colloidal nanoparticles
  • Results of in vitro studies on different cell lines
  • Ethical approval no. 141 from the Sanitary Veterinary and Food Safety Authority Cluj for animal testing (November 13, 2018).

Results dissemination

  • Published papers:
  1. Title: Structural and magnetic characterization of Fe3O4 nanoparticles synthesized at room temperature

Authors: G. Souca, S. Mican, A. Stefancu, V. Chis, R. Tetean, N. Leopold

Journal: STUDIA UBB PHYSICA, Vol. 61 (LXI), 2, 2016 (December), pp. 83‐90

  1. Title: Rho-kinase inhibition ameliorates dasatinib-induced endothelial dysfunction and pulmonary hypertension

Authors: C. Fazakas, C. Nagaraj, D. Zabini, A.G. Vegh, L.M. Marsh, I. Wilhelm, I. Krizbai, H. Olschewski, A. Olschewski, Z. Balint

Journal: Frontiers in Physiology, Vascular Physiology (Front. Physiol., 9:537. 15 May 2018 https://doi.org/10.3389/fphys.2018.00537)

  1. Title: SERS-based quantification of albuminuria in the normal-to-mildly increased range

Authors: A. Stefancu, V. Moisoiu, C. Bocsa, Z. Balint, D.T. Cosma, I.A. Veresiu, V. Chis, N. Leopold, F. Elec

Journal: Analyst (Impact factor: 3.864), Issue 22, 2018; cover article.

  • Scientific publications in review:
  1. Title: Raman imaging-based discrimination between amniotic membrane-derived stem cells and retinal progenitors

Authors: V. Moisoiu, C. Tomuleasa, O. Soritau, M. Aldea, I.S. Florian, A. Stefancu, Z. Diaconeasa, L.F. Leopold, R. Boitor, I. Berindan-Neagoe, V. Chis, I. Notingher, N. Leopold, C.M. Mihu

Journal: Biomedical Optics Express (sent in April, 2017)

  1. Title: Cytotoxicity and photothermal properties of gold nanoparticle assemblies obtained by hydroxylamine reduction

Authors: I.S. Tódor, O.T. Marişca, D. Rugină, Z. Diaconeasa, L.F. Leopold, C. Coman, E. Antonescu, Z. Bálint, L. Szabó, V. Moisoiu, V. Chiş, N. Leopold

Journal: Nanomaterials (sent in April 2018).

  • Conferences
  1. Title: Fast synthesis of gold coated magnetic nanoparticles

Authors: L. Szabó, A. Stefancu, S.r Mican, L.F. Leopold, Z. Bálint, R. Tetean, N. Leopold

Conference: XIVth International Conference on Molecular Spectroscopy. From Molecules to Nano- and Biomaterials (ICMS 2017), September 3–7, 2017, Kraków – Białka Tatrzańska, Poland.

  1. Title: Raman and fluorescence mapping for lipofuscin and norbixin distribution in retinal epithelial cells

Authors: S. Iancu, V. Moisoiu, A. Stefancu, A. Biriș, O. Chakirou, C. Coman, L.F. Leopold, L. Szabo, Z. Balint, N. Leopold

Conference: International Conference on Biomedical Photonics, March 16–17, 2018, Montpellier, France.

  1. Title: SERS on serum, synovial fluid and urine for diagnostic purposes

Authors: V. Moisoiu, A. Stefancu, N. Leopold

Conference: Raman 4 Clinics – Working Group 1 Meeting, Therapeutic monitoring of anti-tumoral drugs and antibiotics in body fluids, March 26–27, 2018, Trieste, Italy.

  1. Title: Iron Oxide-Gold Nanoparticles Functionalised with Glucose as Potential Superparamagnetic Contrast Agents

Authors: S. Iancu, V. Moisoiu, A. Stefancu, A. Biriș, L. Szabo, Z. Balint, N. Leopold

Conference: National Biophysics Conference 2018, September 7–10, 2018, București, Romania

  1. Title: Specific adatoms for anionic and cationic analytes in SERS

Authors: N. Leopold, A. Stefancu, V. Moisoiu, S. Iancu

Conference: SERS Round Table 2018, Novemeber 14-16, 2018, Poltersdorf, Germany

  1. Title: Biofluid analysis by SERS for point-of-care diagnosis

Authors: N. Leopold, A. Stefancu, V. Moisoiu, S. Iancu

Conference: SERS Round Table 2018, Novemeber 14-16, 2018, Poltersdorf, Germany

  1. Title: Assessment of anionic species adsorption onto silver nanoparticles by surface-enhanced Raman scattering (SERS)

Authors: Stefania D. Iancu, Andrei Stefancu, Vlad Moisoiu, Zoltán Bálint, Nicolae Leopold

Conference: ICASS: 3rd International Conference on Applied Surface Science, 17-20 June 2019, Pisa, Italy